Western Blot using anti-Lambda light chain antibody N10/2 (Ab01326) Human spleen (A), human tonsil (B), human thymus (C) and human plasma (D) tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of N10/2 (Ab01326-23.0) at 0.003 µg/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.